Read e-book online Advanced Techniques in Diagnostic Microbiology PDF

By Yi-Wei Tang, Charles W. Stratton

ISBN-10: 0387297413

ISBN-13: 9780387297415

ISBN-10: 0387328920

ISBN-13: 9780387328928

Medical microbiologists are engaged within the box of diagnostic microbiology to figure out no matter if pathogenic microorganisms are found in medical specimens gathered from sufferers with suspected infections. If microorganisms are discovered, those are pointed out and susceptibility profiles, whilst indicated, are made up our minds. in the past 20 years, technical advances within the box of diagnostic microbiology have made consistent and massive development in a number of parts, together with bacteriology, mycology, mycobacteriology, parasitology, and virology. The diagnostic services of recent scientific microbiology laboratories have enhanced speedily and feature increased significantly because of a technological revolution in molecular facets of microbiology and immunology. particularly, speedy suggestions for nucleic acid amplification and characterization mixed with automation and elementary software program have considerably broadened the diagnostic arsenal for the scientific microbiologist. the traditional diagnostic version for scientific microbiology has been labor-intensive and regularly required days to weeks ahead of attempt effects have been on hand. additionally, end result of the complexity and size of such trying out, this provider used to be often directed on the hospitalized sufferer inhabitants. The actual constitution of laboratories, staffing styles, workflow, and turnaround time all were encouraged profoundly via those technical advances. Such adjustments will certainly proceed and lead the sphere of diagnostic microbiology unavoidably to a very smooth self-discipline. complicated suggestions in Diagnostic Microbiology presents a finished and updated description of complex equipment that experience advanced for the prognosis of infectious ailments within the regimen medical microbiology laboratory. The booklet is split into sections. the 1st concepts part covers the foundations and features of innovations starting from swift antigen trying out, to complex antibody detection, to in vitro nucleic acid amplification suggestions, and to nucleic acid microarray and mass spectrometry. adequate house is assigned to hide assorted nucleic acid amplification codecs which are presently getting used broadly within the diagnostic microbiology box. inside of each one strategy, examples are given concerning its program within the diagnostic box. advertisement product details, if to be had, is brought with remark in every one bankruptcy. If numerous attempt codecs can be found for a strategy, target comparisons are given to demonstrate the contrasts in their merits and downsides. the second one functions part presents sensible examples of software of those complicated thoughts in numerous "hot" spots within the diagnostic box. a various workforce of authors offers authoritative and finished details on sequence-based bacterial id, blood and blood product screening, molecular analysis of sexually transmitted ailments, advances in mycobacterial prognosis, novel and quick rising microorganism detection and genotyping, and destiny instructions within the diagnostic microbiology box. we are hoping our readers like this technique-based procedure and your suggestions is extremely favored. we wish to thank the authors who committed their time and efforts to supply their chapters. We additionally thank the employees at Springer Press, specifically Melissa Ramondetta, who initiated the total venture. eventually, we vastly get pleasure from the consistent encouragement of our kinfolk via this lengthy attempt. with out their unwavering religion and entire help, we'd by no means have had the braveness to start this venture.

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2004). Non-invasive tests for the diagnosis of H. pylori infection. Rev Gastroenterol Disorders, 4(1), 1–6. Versalovic, J. (2003). Helicobacter pylori—pathology and diagnostic strategies. Am J Clin Pathol, 119(3), 403–412. , & Fox, J. G. (2003). Helicobacter. In: Murray, P. , Baron, E. , Jorgensen, J. , Pfaller M. , & Yolken, R. , Vol. 1, ASM Press, Washington, DC, pp. 915–928. Warren, J. , & Marshall, B. (1983). Unidentified curved bacilli on gastric epithelium in active chronic gastritis. Lancet, 1(8336), 1273–1275.

A C-13-urea breath test in children with Helicobacter pylori infection: assessment of eradication therapy and follow-up after treatment. J Gastroenterol, 36(9), 606–611. 3 Rapid Antigen Tests SHELDON CAMPBELL AND MARIE L. LANDRY Introduction Immunoassays for the detection of the antigens of microorganisms remain important tools for the diagnosis and management of infectious diseases. Great strides have been made since the introduction of the early precipitation and agglutination assays in increasing the sensitivity, specificity, standardization, and automation of antigen tests (Hage, 1999; Carpenter, 2002; Constantine and Lana, 2003; Peruski and Peruski, 2003).

2002). Diagnosis of Helicobacter pylori in bleeding peptic ulcer patients, evaluation of ureabased tests. Digestion, 66(1), 9–13. Wong, W. , Wong, B. C. , Wong, K. , Fung, F. M. , Lai, K. , Hu, W. H. , Yuen, S. , Leung, S. , Lau, G. K. , Lai, C. , Chan, C. , & Lam, S. K. (2000). C-13-urea breath test without a test meal is highly accurate for the detection of Helicobacter pylori infection in Chinese. Aliment Pharmacol Ther, 14(10), 1353–1358. Wong, W. , Lam, S. , Lai, K. , Chu, K. , Xia, H. H.

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Advanced Techniques in Diagnostic Microbiology by Yi-Wei Tang, Charles W. Stratton


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