By Bhakti Bajpai (auth.), Indu Ravi, Mamta Baunthiyal, Jyoti Saxena (eds.)
The ebook “Advances in Biotechnology” is set fresh advances in a few of the vital fields which are ongoing in sure biotechnological functions. Biotechnology has been really priceless in holding velocity with the calls for of each expanding human inhabitants and in enhancing the standard of human existence. significant biotechnological achievements linked to human welfare were from the fields like genetic engineering; transgenic crops and animals; genomics, proteomics, monoclonal antibodies for the analysis of illness, gene treatment and so forth. Fourteen authoritative chapters written via specialists having adventure in teachers and examine on present advancements and destiny traits in biotechnology were empathized. The ebook presents a close account of varied methodologies utilized in biotechnology i.e. excessive means vectors, DNA sequencing facing subsequent iteration sequencing, Molecular markers, DNA microarray expertise, in addition to Proteomics that experience revolutionized biotechnology with a big selection of purposes. The booklet not just offers a well-founded rationalization of the themes but additionally goals to offer updated experiences of present study efforts, a few considerate discussions at the capability merits and hazards excited about generating biotechnological items and the demanding situations of bringing such items to marketplace. it's going to end up to be an outstanding reference paintings for either academicians and researchers, indicating new beginning issues to younger researchers for brand spanking new initiatives within the box. The publication is meant for biotechnologist, biologist, researchers, lecturers and scholars of Biosciences and Biotechnology.
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Bacterial sample needs 2 to 10 million reads per sample. 6 Applications of DNA Sequencing The Genome sequencing has revolutionized the understanding of treatment and prevention of human diseases at affordable price; genome sequencing has got many more significant applications (Drmanac et al. 2010). 1. DNA sequencing plays vital role in the field of agriculture. The mapping and sequencing of the whole genome of microorganisms has allowed the agriculturists to make them useful for the crops and food plants.
Conversely, due to their high degree of mutability, microsatellites markers are not suitable for analysis and correlation studies of higher taxonomic groups. SSR markers have been useful for genetic variation studies in germplasm collections and are considered to be ideal molecular markers for gene mapping studies (Hearne et al. 1992; Jarne and Lagoda 1996). SSRs are molecular markers used for recombination mapping studies, to verify parental relationships and population genetic studies. They are the only molecular marker to provide information about presence or absence of closely related alleles.
Due to the high degree of variability present in microsatellites, they are preferred in population studies (Smith and Devey 1994) and to distinguish closely related plant cultivars. Simple sequence repeat polymorphism is detected by the use of PCR, in conditions when nucleotide sequences of the flanking regions of the microsatellite are known, or by Southern 32 hybridization using labeled probes. Unlike minisatellites, which also represent tandem repeats, microsatellites consist of short tandem repeat motifs of 1–6 base pair (bp) long units.
Advances in Biotechnology by Bhakti Bajpai (auth.), Indu Ravi, Mamta Baunthiyal, Jyoti Saxena (eds.)