Download e-book for kindle: Animal Cell Culture and Technology (THE BASICS (Garland by Michael Butler

By Michael Butler

ISBN-10: 1859960499

ISBN-13: 9781859960493

This ebook is a wonderful source for college kids and starting medical reaseraches. it's a strong resource of simple equipment and strategies for profitable laboratory telephone tradition systems. many of the present cellphone tradition equipment are coated yet this insurance isn't very distinct. back, very good for newbies.

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Extra resources for Animal Cell Culture and Technology (THE BASICS (Garland Science))

Sample text

A separate master controller that can control several stirrer modules is placed outside the incubator. 11a). The three standard sizes of roller bottles offer a growth surface of 490, 850 or 1750 cm2 and can be filled with 250–500 ml of medium. 11b). On inoculation into the culture medium, the cells attach and grow over the entire inner surface. The bottles are positioned to turn slowly along the long axis at between 5–60 revolutions per hour (rph). The volume of medium added should be just sufficient to provide a shallow covering of the cell monolayer.

The atmosphere of the incubator chamber. The T-flask caps can be adjusted to fit loosely, to allow gas exchange with the environment of the incubator. The caps should be closed tightly once the flasks are removed from the incubator. 6). 10). The stirring should be stable over a long period at a rotation speed of between 10 and 300 rpm. The bottle (or flask) is usually fitted with one or more side arms. These are useful for sampling or as ports for probes or tubing. Spinner bottles can be designed up to a capacity of 5–10 liters.

The use of presterilized and disposable plastic flasks has significantly reduced any contamination arising from the culture vessels. There is no need for an extensive washing process, which is critical to ensure the complete removal of cytotoxic contaminants from glass containers. Whatever material is used, the surface charge density is critical for the attachment of cells. Thus, the physical and chemical treatment of surfaces has a considerable effect on cell adhesion. New borosilicate glass flasks or bottles often show poor cell adherence but this can be improved by washing, sterilization and chemical treatment.

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Animal Cell Culture and Technology (THE BASICS (Garland Science)) by Michael Butler


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